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By Qingming Luo, Lihong V. Wang, Valery V. Tuchin

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4, 26 (2007). J. C. Wilson, Lasers Surg. Med. 38, 384 (2006). Z. Huang, Photodiag. Photodyn. Ther. 3, 71 (2006). M. Biel, Lasers Surg. Med. 38, 349 (2006). G. 3-8. Harwood Academic Publishers, London (1997). M. Loewen, R. Pandey, D. Bellnier, B. Henderson and T. Dougherty, Lasers Surg. Med. 38, 364 (2006). J. Jankun, L. Lilge, A. Douplik, et al. J. Urol. 172, 739 (2004). L. Du, R. M. Busch, et al. Lasers Surg. Med. 38, 427 (2006). Treatment of Age-related Macular Degeneration with Photodynamic Therapy (TAP) Study Group.

37 It was found that hypoxic tumor cells were protected from PDT damage. Therefore, complete explicit dosimetry clearly needs a term describing the oxygen consumption during the PDT session. This is particularly important in the situation where high light fluence and high drug concentration are used. Measurement of oxygen level can be accomplished either using Eppendorf or spectroscopic measurement of absorption coefficient, from which oxy- and deoxy-hemoglobin concentration and drug concentration can be extracted using spectral deconvolution method such as singular value decomposition method.

Nat. Med. 5(10), pp1209-1213 (1999). 2. C. Ince, Crit. Care 9(suppl 4), S13-S19 (2005). 3. DA Beach, C Bustamante, KS Wells, and KM Foucar, Biophys. J 53, pp449-456 (1988). 26 4. 5. 6. R. N. Pittman and B. R. Duling, J. Appl. Phys. 38, 315 (1975). B Lu, SP Morgan, JA Crowe and IM Stockford, Applied Spectroscopy 60: 1157-1166 (2006). A. Serov, B. Steinacher and T. Lasser, Opt. Express. 13, 3681 (2005). cn Laser-based photoacoustic imaging and microwave-based thermoacoustic imaging, combining the advantages of both the high image contrast that results from electromagnetic absorption and the high resolution of ultrasound imaging, could be the next successful generation imaging techniques in biomedical application.

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